Synergistic Effects of Rhizobium and Micronutrients (Molybdenum and Boron) on the Growth, Nodulation, and Yield of Blackgram (Vigna mungo L.)

Authors

  • Shankar Bhandari Tribhuvan University, Institute of Agriculture and Animal Science, Lamjung Campus, Sundarbazar, Lamjung 33600, Gandaki Province, Nepal.
  • Gaman Sharma Valley Krishi Campus, Chapagaun, Lalitpur 44700, Bagmati Province, Nepal. https://orcid.org/0009-0004-9463-5126
  • Sudikshya Shrestha Tribhuvan University, Institute of Agriculture and Animal Science, Lamjung Campus, Sundarbazar, Lamjung 33600, Gandaki Province, Nepal.

DOI:

https://doi.org/10.56946/jspae.v4i2.740

Keywords:

Blackgram, grain yield, integrated nutrient management, nodulation, Rhizobium inoculation

Abstract

Blackgram (Vigna mungo L.) is a vital pulse crop for food and nutritional security in the mid-hills of Nepal, but its productivity remains constrained by poor nutrient management and limited use of Rhizobium inoculants. In particular, deficiencies of essential micronutrients such as boron (B) and molybdenum (Mo), together with underutilization of beneficial Rhizobium, restrict nodulation, nitrogen fixation, and yield potential. To address this issue, a field experiment was conducted at the Agronomy Farm, Lamjung Campus, to assess the combined effects of Rhizobium inoculation and micronutrient supplementation on local blackgram. A split-plot design with three replications was used, where Rhizobium inoculation (inoculated vs. non-inoculated) formed the main plot factor, and seven nutrient treatments were assigned to subplots: control, 250 ppm B, 500 ppm B, 250 ppm Mo, 500 ppm Mo, 250 ppm B + 250 ppm Mo, and 500 ppm B + 500 ppm Mo. Results showed that Rhizobium inoculation significantly improved nodulation, biomass, and yield attributes compared with non-inoculated plots. The combined application of 500 ppm B and 500 ppm Mo with Rhizobium inoculation achieved the highest nodulation, biomass, and seed yield (120.73 g per 2.1 m2). These findings suggest that integrated nutrient management using Rhizobium inoculation with balanced B and Mo supplementation can substantially enhance blackgram productivity, reduce dependence on chemical fertilizers, and contribute to sustainable pulse-based farming systems in Nepal’s mid-hills.

References

Allito, B. B., Ewusi-Mensah, N., Logah, V., & Hunegnaw, D. K. Legume-rhizobium specificity effect on nodulation, biomass production and partitioning of faba bean (Vicia faba L.). Scientific Reports. (2021). 11, 1–11. https://doi.org/10.1038/s41598-021-83235-8

Bhuiyan, M. A. H., Mian, M. H., & Islam, M. S. Bradyrhizobium inoculation. Bangladesh Journal of Agricultural Research. (2008). 33, 449–457. https://doi.org/10.3329/bjar.v33i3.1604

Chaudhary, N., Puri, C., Jaisi, P. N., & Basnet, S. Effect of integrated nutrient management on yield and yield attributing characters of blackgram in Lamahi, Dang. Sustainability in Food and Agriculture. (2020). 1, 106–108. https://doi.org/10.26480/sfna.02.2020.106.108

Chen, W., Li, Y., Shi, G., Fan, G., Tong, F., Liu, L., … & Gao, Y. The role of symbiotic nitrogen-fixing bacteria, Rhizobium and Sinorhizobium, as “bridges” in the rhizosphere of legumes after fomesafen application. Applied Soil Ecology. (2025). 209, 106013. https://doi.org/10.1016/j.apsoil.2025.106013

Dhakal, A. Present status of grain legumes production in Nepal. Food & Agribusiness Management. (2020). 2, 6–9. https://doi.org/10.26480/fabm.01.2021.06.09

Farooq, M., Wahid, A., & Siddique, K. H. M. Micronutrient application through seed treatments – a review. Journal of Soil Science and Plant Nutrition. (2012). 12, 125–142. https://doi.org/10.4067/S0718-95162012000100011

Fazil, K., Hayat, S., Obaid, H., Ashna, A., & Nimgarri, H. Effect of nitrogen and phosphorus on growth and yield of mungbean. Journal of Agriculture and Ecology. (2024). 18, 44–47. https://doi.org/10.58628/jae-2418-107

Ferguson, B. J., Lin, M. H., & Gresshoff, P. M. Regulation of legume nodulation by acidic growth conditions. Plant Signaling and Behavior. (2013). 8. https://doi.org/10.4161/psb.23426

Goyal, R. K., Mattoo, A. K., & Schmidt, M. A. Rhizobial-host interactions and symbiotic nitrogen fixation in legume crops toward agriculture sustainability. Frontiers in Microbiology. (2021). 12, 669404. https://doi.org/10.3389/fmicb.2021.669404

Hossain, A., Quddus, A., Alam, K., Naser, H. M., Anwar, B., Khatun, F., Rashid, H., & Siddiky, A. Application of zinc, boron, and molybdenum in soil increases lentil productivity, nutrient uptake, and apparent balance. Canadian Journal of Soil Science. (2021). 101, 113–124. https://doi.org/10.1139/cjss-2019-0141

Hussey, R. S., & Barker, K. R. Influence of nematodes and light sources on growth and nodulation of soybean. Journal of Nematology. (1976). 8, 48–52. https://pmc.ncbi.nlm.nih.gov/articles/PMC2620149/

Jat, S., Dangi, K. L., & Kumhar, B. L. Constraints in adoption of improved cultivation practices of black gram. International Journal of Current Microbiology and Applied Sciences. (2017). 6, 1820–1824. https://doi.org/10.20546/ijcmas.2017.605.198

Johansen, C., Musa, A. M., Kumar Rao, J. V. D. K., Harris, D., Shahidullah, A. K. M., & Lauren, G. J. Seed priming with molybdenum alleviates molybdenum deficiency and poor nitrogen fixation of chickpea in acid soils of Bangladesh and India. Proceedings of the 18th World Congress of Soil Science. (2006).

Joshi, K. D., & Sthapit, B. R. Genetic variability and possible genetic advance for sterility tolerance in wheat (Triticum aestivum L.) through breeding. In Sterility in wheat in subtropical Asia: extent, causes and solutions. Proceedings of a workshop. (1995). 135–143.

Kathyayani, K., Singh, R., & Chhetri, P. Effect of levels of boron and molybdenum on economics of blackgram (Vigna mungo L.) cultivation. International Journal of Chemical Studies. (2021). 9, 1945–1947. https://doi.org/10.22271/chemi.2021.v9.i1aa.11507

Kavitha, S., & Srimathi, P. Micro-nutrients seed priming and Rhizobium coating on seed vigour, crop growth and seed yield in blackgram (Vigna mungo L.). Legume Research – An International Journal. (2022). 45, 1559–1563. https://doi.org/10.18805/LR-4488

Khanal, N., Harris, D., & Sherpa, L. T. Legume viral diseases in Nepal. Journal of Plant Protection Society. (2020). 6, 255–282.

Kuzbakova, M., Khassanova, G., Oshergina, I., Ten, E., Jatayev, S., Yerzhebayeva, R., & Shavrukov, Y. Height to first pod: A review of genetic and breeding approaches to improve combine harvesting in legume crops. Frontiers in Plant Science. (2022). 13, 948099. https://doi.org/10.3389/fpls.2022.948099

Liu, P., Yang, Y. S., Xu, G. D., Fang, Y. H., Yang, Y. A., & Kalin, R. M. The effect of molybdenum and boron in soil on the growth and photosynthesis of three soybean varieties. Plant, Soil and Environment. (2005). 51, 197–205. https://doi.org/10.17221/3574-PSE

Mahesh, K., Umesha, C., Karthik, B., Spandana, R., & Priyadharshini, A. S. Performance of phosphorus and molybdenum levels on growth and economics of blackgram (Vigna mungo L.). The Pharma Innovation Journal. (2021). 10, 867–869. http://www.thepharmajournal.com

Mahilane, C., Singh, V., Kumar, M., & Singh, A. C. Response of different levels of zinc and molybdenum on growth and yield of blackgram (Vigna mungo L.) under agro-climatic east Uttar Pradesh. Journal of Pharmacognosy and Phytochemistry. (n.d.). https://jpds.co.in/wp-content/uploads/2019/07/17.-Chhatrapati-Mahilane-1132.pdf

Mir, A., Lal, S., Salmani, M., Abid, M., & Khan, I. Growth, yield and nutrient content of blackgram (Vigna mungo) as influenced by levels of phosphorus, sulphur and phosphorus solubilizing bacteria. SAARC Journal of Agriculture. (2013). 11, 1–6. https://doi.org/10.3329/sja.v11i1.18370

MoALD. Statistical information of Nepalese agriculture. Ministry of Agriculture and Livestock Development, Nepal. (2023).

Movalia, J., Donga, S., & Parmar, K. B. Effect of boron and molybdenum on summer green gram (Vigna radiata L.) under medium black calcareous soils: A review. Proceedings of the National Conference on Innovations in Biological Sciences (NCIBS). (2020). http://dx.doi.org/10.2139/ssrn.3585103

Myageri, P. V., & Dawson, J. Effect of phosphorus and boron levels on growth and yield of lentil (Lens culinaris L.). International Journal of Plant & Soil Science. (2022). 34, 504–510. https://doi.org/10.9734/ijpss/2022/v34i2031181

Naznin, F., Hossain, M. A., Khan, M. A., Islam, M. A., & Rahman, A. K. M. H. Effect of boron on growth, yield, and nutrient accumulation in black gram. The Agriculturists. (2020). 18, 34–43.

Niraula, S., & Sharma, R. Performance of black gram (Vigna mungo L. Hepper) under different levels of phosphorus at Bardiya, Nepal. Agronomy Journal of Nepal. (2024). 8, 180–188. https://doi.org/10.3126/ajn.v8i1.70858

Pandey, S. P., Yadav, C. R., Sah, K., Pande, S., & Joshi, P. K. Legumes in Nepal. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). (2000). http://oar.icrisat.org/id/eprint/3460

Prabhaharan, J., Kannan, P., Arunachlam, P., & Prabukumar, G. Response of Blackgram (Vigna mungo L.) to multi-micronutrient mixtures under rainfed Alfisol. Journal of the Indian Society of Soil Science. (2014). https://epubs.icar.org.in/index.php/JISSS/article/view/43566

Raju, R. S., Rani, Y. A., Sreekanth, B., & Jyothula, D. P. B. Influence of boron, molybdenum and nickel on total dry matter, partitioning and yield of blackgram (Vigna mungo L. Hepper). International Journal of Current Microbiology and Applied Sciences. (2019). 8, 512–521. https://doi.org/10.20546/ijcmas.2019.812.068

Rutkowska, B., Szulc, W., Spychaj-Fabisiak, E., & Pior, N. Prediction of molybdenum availability to plants in differentiated soil conditions. Plant, Soil and Environment. (2017). 63, 531–536. https://doi.org/10.17221/616/2017-PSE

Sadiq, M., Rahim, N., Iqbal, M. A., Alqahtani, M. D., Tahir, M. M., Majeed, A., & Ahmed, R. Rhizobia inoculation supplemented with nitrogen fertilization enhances root nodulation, productivity, and nitrogen dynamics in soil and black gram (Vigna mungo L.). Land. (2023). 12, 714. https://doi.org/10.3390/land12071434

Sharmila, S., Pandian, P. S., Indirani, R., & Subramanian, E. Influence of boron on growth attributes, yield of black gram and available boron status in Typic chromustert. International Journal of Chemical Studies. (2020). 8, 2291–2294. https://doi.org/10.22271/chemi.2020.v8.i6ag.11115

Sinclair, T. R., & Vadez, V. The future of grain legumes in cropping systems. Crop and Pasture Science. (2012). 63, 501–512. https://doi.org/10.1071/CP12128

Singh, S. P. Effect of micronutrients on nodulation, growth, yield and nutrient uptake in black gram (Vigna mungo L.). Annals of Plant and Soil Research. (2017). 19, 66–70.

Tahir, M., Sher, A., & Majeed, M. A. Effect of molybdenum on yield and quality of black gram (Vigna mungo L.). Pakistan Journal of Life and Social Sciences. (2014). 12, 101–105.

Thilakarathna, M. S., Chapagain, T., Ghimire, B., Pudasaini, R., Tamang, B. B., Gurung, K., Choi, K., Rai, L., Magar, S., Bk, B., Gaire, S., & Raizada, M. N. Evaluating the effectiveness of Rhizobium inoculants and micronutrients as technologies for Nepalese common bean smallholder farmers in the real-world context of highly variable hillside environments and indigenous farming practices. Agriculture. (2019). 9, 20. https://doi.org/10.3390/agriculture9010020

Tripathi, S., Kumar, S., Kumar, M., Kumar, A., Dhyani, B. P., & Kumar, Y. Effect of Rhizobium inoculation methods on growth, nodulation and yield of black gram (Vigna mungo L.). International Journal of Current Microbiology and Applied Sciences. (2021). 10, 1588–1598. https://doi.org/10.20546/ijcmas.2021.1001.185

van Kessel, C., & Hartley, C. Agricultural management of grain legumes: Has it led to an increase in nitrogen fixation? Field Crops Research. (2000). 65, 165–181. https://doi.org/10.1016/S0378-4290(99)00085-4

Veer, D., Diwakar, S. K., Tomar, S. P., Mohasin, M., & Singh, R. P. Effect of Rhizobium, phosphorus and nitrogen on growth, yield and yield attributes of summer black gram. The Pharma Innovation Journal. (2021). 11, 1192–1195.

Veer, D., Habib, K., & Kumar, K. Response of black gram (Vigna mungo L. Hepper) to Rhizobium, phosphorus and nitrogen for sustainable agriculture: A mini review. International Journal of Plant and Environment. (2022). 8, 81–86. https://doi.org/10.18811/ijpen.v8i01.10

Vijila, K., & Jebaraj, S. Studies on the improvement of Rhizobium–green gram (Vigna radiata L.) symbiosis in low nutrient, acid stress soils. Legume Research. (2008). 31, 126–129.

Downloads

Published

2025-09-30
CITATION
DOI: 10.56946/jspae.v4i2.740

How to Cite

Bhandari, S., Sharma, G., & Shrestha, S. (2025). Synergistic Effects of Rhizobium and Micronutrients (Molybdenum and Boron) on the Growth, Nodulation, and Yield of Blackgram (Vigna mungo L.). Journal of Soil, Plant and Environment, 4(2), 95–107. https://doi.org/10.56946/jspae.v4i2.740

Issue

Section

Article