Synergistic Effects of Bacterium ZM12 and Vermicompost on Groundnut Yield, Quality, and Nutrient Uptake
DOI:
https://doi.org/10.56946/jspae.v4i1.678Keywords:
Composition, endophytic bacteria, peanut, pure colony, rhizosphereAbstract
Peanut cultivation in nutrient-poor soils often relies heavily on chemical fertilizers, leading to soil degradation, reduced productivity, and lower profitability for farmers. To address this issue, a sustainable alternative combining organic amendments and beneficial microbes was evaluated. The research objective is to assess the influences of vermicompost addition and inoculant with Bacterium strain ZM12 on the growth, yield, and quality of peanuts. Bacterium strain ZM12 was isolated on YMA medium and identified using 16S rRNA gene sequencing. Field research was conducted using a factorial design with two factors: (i) inoculation vs. non-inoculation with strain ZM12 and (ii) three rates of vermicompost (0, 5, and 10 t ha-1), resulting in six treatments with four replications. The combined application of 10 t ha-1 vermicompost and ZM12 inoculation significantly enhanced peanut protein content and growth performance. Peanut yield increased by 38.4% with 10 t ha-1 vermicompost compared to the control (0 t ha-1), and ZM12 inoculation alone improved yield by 12.9% compared to non-inoculated plots. These improvements were attributed not only to biological nitrogen fixation but also to the plant growth-promoting effects of strain ZM12. The study demonstrates an effective strategy for improving peanut productivity and soil fertility while reducing dependence on chemical fertilizers. However, future work should include the isolation and testing of additional beneficial microbial strains to further enhance the sustainability of this approach.
References
Abdel-Mouty, M. M., Mahmoud, A. R., EL-Desuki, M., & Rizk, F.A. Yield and fruit quality of Eggplant as Affected by organic and Mineral Fertilizers Application. Research Journal of Agriculture and Biological Sciences, 7. (2011). 196-202.
Aktar, S., Quddus, M. A., Hossain, M. A., Parvin, S., & Sultana, M. N. Effect of integrated nutrient management on the yield, yield attributes and protein content of lentil. Bangladesh Journal of Agricultural Research. 44(3). (2019). 525-536. https://doi.10.3329/bjar.v44i3.43483
Albuena, D., Tui, S. H. K., Erenstein, O., Teufel, N., Duncan, A., & Abdoulaye, T. Identifying determinants, pressures and trade-offs of crop residue use in mixed smallholder farms in Sub-Saharan Africa and South Asia. Agricultural Systems, 134. (2015). 107–118. https://doi.10.1016/j.agsy.2014.05.013.
Alfandi, S., & Wahyuni, Nisa, W.W. Effect of Rhizobium Inoculum and Liquid Organic Fertilizer on Growth and Yield of Peanut (Arachis Hypogaea L.) CV. Takar-2. IOP Conf. Ser.: Earth Environ. Sci., 391. (2019). 012004. https://doi.org.10.1088/1755-1315/391/1/012004.
Ali, O. A., El-Tahlawy, Y. A. and Abdel-Gwad, S. A. Impact of Compost Tea Types Application on Germination, Nodulation, Morphological Characters and Yield of Two Lentil Cultivars. Egyptian Journal of Agronomy, 1-19. (2018). 1-19. https://doi.10.21608/AGRO.2018.5678.1126.
Amos, H., Voncir, N., Fagam, A.S., & Garba, A. Effect of cattle manure on the growth and yield performance of vegetable maize (Zea mays Saccharata) varieties under irrigation. Agricultural Science, 2(4) (2015). 319-323. https://doi.org/10.22146/ipas.72990
An-Dong Gong, Fei-Yan Dong, Meng-Jun Hu, Xian-Wei Kong, Fen-Fen Wei, Shuang-Jun Gong, Yi-Mei Zhang, Jing-Bo Zhang, Ai-Bo W. U., & Yu-Cai Lia. Antifungal activity of volatile emitted from Enterobacter asburiae Vt-7 against Aspergillus flavus and aflatoxins in peanuts during storage. Food Control, 106. (2019). 106718. https://doi.org/10.1016/j.foodcont.2019.106718.
Arancon, N. Q., Edwards, C. A., Atiyeh, R., & Metzger, J. D. Effects of vermicomposts produced from food waste on the growth and yields of greenhouse peppers. Bioresour Technology. 93(2). (2004).139-44. https://doi. 10.1016/j.biortech.2003.10.015.
Argaw, A. Organic and inorganic fertilizer application enhances the effect of Bradyrhizobium on nodulation and yield of peanut (Arachis hypogea L.) in nutrient depleted and sandy soils of Ethiopia. International Journal of Recycling of Organic Waste in Agriculture, 6. (2017). 219–231. https://doi.10.1007/s40093-017-0169-3.
Asnake, B., & Fassil, A. Isolation,Identification and Characterization Effective Rhizobium Species Nodulating Mung Bean (Vigna radiata) from some places of North Shewa. International Journal - Earth & Environmental Sciences, 20 (1). (2019). 5556026. https://doi.10.19080/IJESNR.2019.20.556026
Baber, M., Fatima, M., Rameesha, A., Muther, M. Q., Sidra, N., Muhammad, K. H., & Tahir, N. Weed rhizosphere: a source of novel plant growth promoting rhizobacteria (PGPR). International Journal of Biosciences, 13(1). (2018). 223-233. http://doi.org/10.12692/ijb/13.1.223-233.
Bakker, P. A. H. M., Ran, L. X., Pieterse, C. M. J., & Loon, L. C. Understanding the involvement of rhizobacteria-mediated induction of systemic resistance in biocontrol of plant diseases. Canadian Journal of Plant Pathology, 25. (2003). 5–9. https://doi.10.1080/07060660309507043.
Brady, N. C. The Nature and Properties of Soils. Macmillan Publishing Co., Inc. Doran, J.W. and Parkin, T.B. 1996. Quantitative Indicators of Soil Quality: A Minimum Data Set. Soil Science Society of America Special Publication No. 49, SSSA, Madison, Wisconsin, USA. (1988).
Camargo, A. C., Regitano, M. A. B., Rasera, G. B., Canniatti-Brazaca, S. G., Prado-Silva, L., Alvarenga, V. O., Sant'Ana, A. S., & Shahidi, F. Phenolic acids and flavonoids of peanut by-products: Antioxidant capacity and antimicrobial effects. Food Chemistry, 237. (2017).538–544. https://doi.org/10.1016/j.foodchem.2017.05.046
Chibeba, A. M., Kyei-Boahen, S., de Fátima Guimarães, M., Nogueira, M. A., & Hungria, M. Isolation, characterization and selection of indigenous Bradyrhizobium strains with outstanding symbiotic performance to increase soybean yields in Mozambique. 246. (2017). 291-305. https://doi.org/10.1016/j.agee.2017.06.017
Choudhary, R. C., Bairwa, H. L., Kumar, U., Javed, T., Asad, M., Lal, K., Mahawer, L. N., Sharma, S. K., Singh, P., Hassan, M. M., Abo-Shosha, A. A., Rajagopal, R., Abdelsalam, N. R. Influence of organic manures on soil nutrient content, microbial population, yield and quality parameters of pomegranate (Punica granatum L.) cv. Bhagwa. PLoS One, 17(4). (2022). e0266675. https://doi.10.1371/journal.pone.0266675.
Chuong, N. V. The impact of bacillus sp. NTLG2-20 and reduced nitrogen fertilization on soil properties and peanut yield. Communications in Science and Technology, 9(1). (2024). 112-120. https://doi.org/10.21924/cst.9.1.2024.1423
Chuong, N. V., & Tri, T. L. K. Enhancing soil fertilizer and peanut output by utilizing endophytic bacteria and vermicompost on arsenic-contaminated soil. International Journal of Agriculture and Biosciences, 13(4). (2024). 596-602. https://doi.org/10.47278/journal.ijab/2024.145
Chuong, N. V., Nguyen Ngoc Phuong, T., & Nguyen Van, T. Nitrogen fertilizer use reduction by two endophytic diazotrophic bacteria for soil nutrients and corn yield. Communications in Science and Technology, 9(2). (2025). 348-355. https://doi.org/10.21924/cst.9.2.2024.1527
Deshwal, V. K., & Chaubey, A. Isolation and characterization of Rhizobium leguminosarum from root nodule of Pisum sativum L. Journal of Academia and Industrial Research, 2(8). (2014). 464-467. https://doi.10.21608/AGRO.2018.5678.1126
Drinkwater, L. E., Schipanski, M., Snapp, S., & Jackson, L. E. Ecologically based nutrient management in Agricultural Systems: Agroecology and Rural Innovation for Development: Second Edition, eds S. Snapp, B. Pound (London: Elsevier Inc), (2017). 203–257. https://doi.10.1016/B978-0-12-802070-8.00007-4.
Erenstein, O., Gérard, B., & Tittonell, P. Biomass use trade-offs in cereal cropping systems in the developing world: overview. Agricultural Systems, 134. (2015). 1–5. https://doi.10.1016/j.agsy.2014.12.001.
Foyer, C. H., Lam, H. M., Nguyen, H. T., Siddique, K. H. M., Varshney, R. K., & Colmer, T. D. Neglecting legumes has compromised human health and sustainable food production. Nature Plants, 2. (2026). 1–10. https://doi.10.1038/NPLANTS.2016.112
Hameed, S., Yasmin, S., Malik, K. A., Zafar, Y., & Hafeez, F. Y. Rhizobium, Bradyrhizobium and Agrobacterium strains isolated from cultivated legumes. Biology and Fertility of Soils. 39(3). (2004). 179-185. https://doi.org/10.1007/s00374-003-0697-z
Hamza, M., Abbas, M., Abd Elrahman, A., Helal, M., & Shahba, M. Conventional versus Nano Calcium Forms on Peanut Production under Sandy Soil Conditions. Agriculture, 11. (2021). 767. https://doi.org/10.3390/agriculture11080767
Hossain, A., Gunri, S. K., Barman, M., Sabagh, A. E., & da Silva, J. A. T. Isolation, characterization and purification of Rhizobium strain to enrich the productivity of groundnut (Arachis hypogaea L.). Open Agriculture, 4(1). (2019). 400-409. https://doi.org/10.1515/opag-2019-0040.
Janila, P., Nigam, S. N., Pandey, M. K., Nagesh, P. and Varshney, R. K. Groundnut improvement: Use of genetic and genomic tools. Frontiers in Plant Sciences. 4. (2013). 23. https://doi.org/10.3389/fpls.2013.00023.
Jordan, D. Rhizobaceae. In: Bergey's Manual of Systematic Bacteriology, 234-256, (D.P. Hendricks et al., eds). Orient Longman, New York. (1984).
Kim, Sang Yeob Lee, Seong Ho Ahn, Ji Hee Han & Jin Woo Park. Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination. The Plant Pathology Journal, 36(3). (2020). 244–254. https://doi.10.5423/PPJ.OA.11.2019.0283
Mahesh, K., Poonam, S. A., & Singh, R.P. Plant growth promoting microbes: Diverse roles for sustainable and ecofriendly agriculture,Energy Nexus,7,2022,100133. https://doi.org/10.1016/j.nexus.2022.100133.
Michel, D., Claude, B., Antoine, C., Rolland, M., Jean-Pierre, G., Didier, R. 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. Journal of Clinical Microbiology Journal, 38. (2000). 3623-3630. https://doi.10.1128/jcm.38.10.3623-3630.2000
Mir, M.I., Nagabhushanam, B., Quadriya, H., Kumar, B. K., & Hameeda, B. Morphological, biochemical and intrinsic antibiotic resistance of rhizobia isolated from root and stem nodules of various leguminous plants. Plant Cell Biotechnology and Molecular Biology, 21(71). (2020). 126-138.
Mthiyane, P., Aycan, M., & Mitsui, T. Integrating Biofertilizers with Organic Fertilizers Enhances Photosynthetic Efficiency and Upregulates Chlorophyll-Related Gene Expression in Rice. Sustainability, 16(21). (2024). 9297. https://doi.org/10.3390/su16219297.
Muñoz, V, Ibañez, F, Tonelli, M.L., Valetti, L., Anzuay ,M. S., & Fabra, A. Phenotypic and phylogenetic characterization of native peanut Bradyrhizobium isolates obtained from Córdoba, Argentina. Systematic and Applied Microbiology, 34(6). (2011). 446-52. doi. https://doi.10.1016/j.syapm.2011.04.007
Nguyen, V. C., Le, M. T., Tran, L. K. T., Nguyen, N. P. T. Ho, T. T., & Nguyen, T. T. D. Efficient evaluation of E. asburiae, K. quasipneumoniae and E. cloacae combination with vermicompost on growth and yield of groundnut, Seybold report, 18. (2023). 862-1870. https://doi.10.17605/OSF.IO/P879S
Park, M., Kim, C., Yang, J., Lee, H., Shin, W., Kim, S., Sa, T. Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea. Microbiological Research, 160(2). (2005). 127-133. https://doi.10.1016/j.micres.2004.10.003
Rahimabadi, R. E., Ansari, M. H., & Nematollahi, A. R. Influence of cow manure and its vermicomposting on the improvement of grain yield and quality of rice (Oryza sativa L.) in Field conditions. Applied Ecology and Environmental Research, 6(1). (2017). 97-110. http://dx.doi.org/10.15666/aeer/1601_097110
Rao, D. L. N. Recent advances in biological nitrogen fixation in agricultural systems. Proceedings of the Indian National Science Academy, 80(2). (2014). 359-378. https://doi.10.16943/PTINSA/2014/V80I2/55114.
Rehman, S. u., De Castro, F., Aprile, A., Benedetti, M., & Fanizzi, F. P. Vermicompost: Enhancing Plant Growth and Combating Abiotic and Biotic Stress. Agronomy, 13(4). (2023). 1134. https://doi.org/10.3390/agronomy13041134.
Singh, B., Kaur, R., & Singh, K. Characterization of Rhizobium strain isolated from the roots of Trigonella foenumgraecum (fenugreek). African Journal of Biotechnology, 7(20), (2008). 3671-3676.
Singh, P., Singh, R. K., Li, H. B., Guo, D. J., Sharma, A., Lakshmanan, P., Malviya, M. K., Song, X. P., Solanki, M. K., Verma, K. K., Yang, L. T., Li, Y. R. Diazotrophic Bacteria Pantoea dispersa and Enterobacter asburiae Promote Sugarcane Growth by Inducing Nitrogen Uptake and Defense-Related Gene Expression. Frontiers in microbiology, 11. (2021). 600417. https://doi.org/10.3389/fmicb.2020.600417.
Snapp, S., Rahmanian, M., Batello, C. Pulse Crops for Sustainable Farms in Sub-Saharan Africa. Rome: FAO, (2018). https://doi.10.18356/6795bfaf-en.
Thierfelder, C., Chisui, J. L., Gama, M., Cheesman, S., Jere, Z. D., & Trent Bunderson, W. Maize-based conservation agriculture systems in Malawi: Long-term trends in productivity. Field Crops Research, 142. (2013). 47–57. https://doi.10.1016/j.fcr.2012.11.010.
Thin, K. K., He, S. W., Wang, X., Wang, Y., Rong, M., Han, J. G., & Zhang, X. Rhizobium rhizolycopersici sp. nov., isolated from the rhizosphere soil of tomato plants in China. Current Microbiology. 78(2). (2021). 830-836. https://doi.10.1007/s00284-020-02323-6
Tittonell, P., Gérard, B., & Erenstein, O. Tradeoffs around crop residue biomass in smallholder crop-livestock systems – What 's next. Agric. Syst. 134. (2015). 119–128. https://doi.10.1016/j.agsy.2015.02.003.
Trang, K. B and Nguyen, V. C. Effects of Rice Husk Biochar, Sawdust and Priestia Aryabhattai M2C on The Productivity and Arsenic Accumulation of Soybeans. Xi'an ShiyouDaxueXuebao (ZiranKexue Ban)/ Journal of Xi'an Shiyou University, Natural Sciences, 66(01). (2023). 1-12. https://doi.org/10.17605/OSF.IO/V3MBP
Trang, N. N. P., & Chuong, N. V. The enhancement of soil fertility and baby maize output by Streptomyces panayensis and vermicompost. Eurasian Journal of Soil Science, 14(2). (2025). 140-148. https://doi.org/10.18393/ejss.1630363
Tyagi, A., Kumar, V., Tomar, A. Isolation, identification, biochemical and antibiotic sensitivity characterization of Rhizobium strains from Vigna mungo (L.) Hepper, Cicer arietinum (L.) and Vigna radiata (L.) Wilczek in Muzaffarnagar, Uttar Pradesh India. International Journal of Current Microbiology and Applied Sciences, 6(12). (2017). 2024-2035. https://doi.org/10.20546/ijcmas.2017.612.233.
Upasana, B., Shipra, M., Sarita, J., & Dalal, A. S. A study of isolation and identification of bacteria from lake water in and around Udaipur, Rajasthan. Journal of Family Medicine and Primary Care, 9(2). (2020). 751–754. https://doi.org/10.4103/jfmpc.jfmpc_1032_19
Vincent, J. M. (A Manual for the Practical Study of Root Nodule Bacteria’, Blackwell, Oxford and Edinburgh, (1970). 164 p.
Yarza, P., Yilmaz, P., Pruesse, E., Glöckner, F. O., Ludwig, W., Schleifer, K. H., & RossellóMóra, R. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nature Reviews Microbiology, 12(9). (2014). 635-645. https://doi.10.1038/nrmicro3330.
Zahran, H. H. Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiology and Molecular Biology Reviews. 63(4). (1999). 968-989. https://doi.10.1128/MMBR.63.4.968-989.1999.
Zhang, M., Liu, Y., Wei, Q., Liu, L., Gu, X., Gou, J., & Wang, M. Ameliorative Effects of Vermicompost Application on Yield, Fertilizer Utilization, and Economic Benefits of Continuous Cropping Pepper in Karst Areas of Southwest China. Agronomy, 13(6). (2023). 1591. https://doi.org/10.3390/agronomy13061591
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